biotinylated mal ii (Vector Laboratories)
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Biotinylated Mal Ii, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 408 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maackia+amurensis+lectin+ii/Biotinylated+Maackia+Amurensis+Lectin+II+(MAL+II)/bio_rxiv__64898__2026__04__15__718752-82-4-7
Average 96 stars, based on 408 article reviews
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Staining:Article Title: Air-liquid interface model for influenza aerosol exposure in vitro Article Snippet: .. Cells were stained with the following primary antibodies: Bioprocessing:Article Title: ST6Gal1 influences the esophageal squamous cell carcinoma resistance to IFN-γ by regulating the expression of IFN-γ receptor 1. Article Snippet: ST6Gal1 ( 2,6-sialyltransferase1), the primary glycosyltransferase catalyzing 2,6-sialylation of N-glycans, plays a pivotal role in the progression of various human cancers.. However, the role of ST6Gal1-mediated 2,6-sialylation in human esophageal squamous cell carcinoma (ESCC) remains poorly understood.. In this study, we established ST6Gal1 knockdown (Kyse450-KD) and overexpressed (Kyse150-OE) ESCC cell models to investigate its functional significance. Immunodetection:Article Title: Engineering glycosyltransferases into glycan binding proteins using a mammalian surface display platform Article Snippet: Recombinant human ST3Gal1 (rhST3Gal1, Product code: 6905-GT-020), Recombinant human P-selectin Fc chimera (P-selectin-Fc, Product code: 137-PS-050), sheep anti-hGCNT1 IgG (Product code: AF7248-SP) and HRP donkey anti-sheep H + L IgG (Product code: HAF016) were from R&D Systems (Minneapolis, MN). .. Unconjugated Peanut Agglutinin (PNA, Product code: L-1070), Blocking Assay:Article Title: Glycoprotein G enables HSV-2 neuroinvasion and provides protection as a glycosylated vaccine antigen Article Snippet: Lectin blot was performed by loading, 2 μg each of EXCT4-mgG-2, EXCT4-mgG-2(−N−O), EXCT4-mgG-2(-N), EXCT4-mgG-2(-O) and EXCT4-mgG-2(-SA) to a NuPAGE Bis-Tris 4-12% gel (Invitrogen) and separated using MOPS buffer (Invitrogen) at 200 V. The separated proteins were transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 μm, (Merck Millipore)) using the SemiDry Transblot SD (Bio-Rad Laboratories). .. Membranes were blocked with block buffer (2% BSA Factor V (Sigma-Aldrich) and 0.1% Tween-20 (VWR chemicals) in PBS (Medicago) followed by incubation with biotinylated lectins; 3 μg/mL Concavalin A (Con A) (Vector Laboratories), 3 μg/mL Jacalin (Vector Laboratories) or 5 μg/mL Incubation:Article Title: Glycoprotein G enables HSV-2 neuroinvasion and provides protection as a glycosylated vaccine antigen Article Snippet: Lectin blot was performed by loading, 2 μg each of EXCT4-mgG-2, EXCT4-mgG-2(−N−O), EXCT4-mgG-2(-N), EXCT4-mgG-2(-O) and EXCT4-mgG-2(-SA) to a NuPAGE Bis-Tris 4-12% gel (Invitrogen) and separated using MOPS buffer (Invitrogen) at 200 V. The separated proteins were transferred to a polyvinylidene difluoride membrane (Immobilon-FL 0.45 μm, (Merck Millipore)) using the SemiDry Transblot SD (Bio-Rad Laboratories). .. Membranes were blocked with block buffer (2% BSA Factor V (Sigma-Aldrich) and 0.1% Tween-20 (VWR chemicals) in PBS (Medicago) followed by incubation with biotinylated lectins; 3 μg/mL Concavalin A (Con A) (Vector Laboratories), 3 μg/mL Jacalin (Vector Laboratories) or 5 μg/mL Article Title: Exploring influenza A virus receptor distribution in the lactating mammary gland of domesticated livestock and in human breast tissue. Article Snippet: Slides were then blocked with 1× Carbo-Free blocking solution (Vector Laboratories Inc.) for 32 min, followed by a Streptavidin/Biotin Blocking Kit (Vector Laboratories Inc.) with a separate application for 12 min each. .. We incubated the sections for 4 h (fluorescent) or 1 h (chromogenic) at room temperature (RT) with one of the 3 lectins (Sambucus nigra lectin [SNA], Article Title: Gne deletion in adult mice can cause thrombocytopenia, anemia, myopathy, bleeding, and death. Article Snippet: .. Biotinylated peanut agglutinin (PNA, B-1075-5), erythrina cristagalli lectin (ECA, B-1145-5), sambucus nigra lectin (SNA, B-1305-2), and Concentration Assay:Article Title: Exploring influenza A virus receptor distribution in the lactating mammary gland of domesticated livestock and in human breast tissue. Article Snippet: Slides were then blocked with 1× Carbo-Free blocking solution (Vector Laboratories Inc.) for 32 min, followed by a Streptavidin/Biotin Blocking Kit (Vector Laboratories Inc.) with a separate application for 12 min each. .. We incubated the sections for 4 h (fluorescent) or 1 h (chromogenic) at room temperature (RT) with one of the 3 lectins (Sambucus nigra lectin [SNA], |
